Comparison of Carbonic Anhydrases for CO2 Sequestration

Autor(en)
Franziska Steger, Johanna Reich, Werner Fuchs, Simon K-M R Rittmann, Georg M Gübitz, Doris Ribitsch, Günther Bochmann
Abstrakt

Strategies for depleting carbon dioxide (CO2) from flue gases are urgently needed and carbonic anhydrases (CAs) can contribute to solving this problem. They catalyze the hydration of CO2 in aqueous solutions and therefore capture the CO2. However, the harsh conditions due to varying process temperatures are limiting factors for the application of enzymes. The current study aims to examine four recombinantly produced CAs from different organisms, namely CAs from Acetobacterium woodii (AwCA or CynT), Persephonella marina (PmCA), Methanobacterium thermoautotrophicum (MtaCA or Cab) and Sulphurihydrogenibium yellowstonense (SspCA). The highest expression yields and activities were found for AwCA (1814 WAU mg−1 AwCA) and PmCA (1748 WAU mg−1 PmCA). AwCA was highly stable in a mesophilic temperature range, whereas PmCA proved to be exceptionally thermostable. Our results indicate the potential to utilize CAs from anaerobic microorganisms to develop CO2 sequestration applications.

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie
Externe Organisation(en)
Universität für Bodenkultur Wien, acib – Austrian Centre of Industrial Biotechnology
Journal
International Journal of Molecular Sciences
Band
23
Anzahl der Seiten
13
ISSN
1422-0067
DOI
https://doi.org/10.3390/ijms23020957
Publikationsdatum
01-2022
Peer-reviewed
Ja
ÖFOS 2012
208003 Umweltbiotechnologie
Schlagwörter
ASJC Scopus Sachgebiete
Molecular Biology, Spectroscopy, Catalysis, Inorganic Chemistry, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/comparison-of-carbonic-anhydrases-for-co2-sequestration(fccceb64-6bf5-489b-ba43-90324ba1d3a6).html