Comparison of Carbonic Anhydrases for CO2 Sequestration

Autor(en)

Franziska Steger, Johanna Reich, Werner Fuchs, Simon K-M R Rittmann, Georg M Gübitz, Doris Ribitsch, Günther Bochmann

Abstrakt

Strategies for depleting carbon dioxide (CO2) from flue gases are urgently needed and carbonic anhydrases (CAs) can contribute to solving this problem. They catalyze the hydration of CO2 in aqueous solutions and therefore capture the CO2. However, the harsh conditions due to varying process temperatures are limiting factors for the application of enzymes. The current study aims to examine four recombinantly produced CAs from different organisms, namely CAs from Acetobacterium woodii (AwCA or CynT), Persephonella marina (PmCA), Methanobacterium thermoautotrophicum (MtaCA or Cab) and Sulphurihydrogenibium yellowstonense (SspCA). The highest expression yields and activities were found for AwCA (1814 WAU mg−1 AwCA) and PmCA (1748 WAU mg−1 PmCA). AwCA was highly stable in a mesophilic temperature range, whereas PmCA proved to be exceptionally thermostable. Our results indicate the potential to utilize CAs from anaerobic microorganisms to develop CO2 sequestration applications.

Organisation(en)

Department für Funktionelle und Evolutionäre Ökologie

Externe Organisation(en)

Universität für Bodenkultur Wien, acib – Austrian Centre of Industrial Biotechnology

Journal

International Journal of Molecular Sciences

Band

23

Anzahl der Seiten

13

ISSN

1422-0067

DOI

https://doi.org/10.3390/ijms23020957

Publikationsdatum

01-2022

Peer-reviewed

Ja

ÖFOS 2012

208003 Umweltbiotechnologie

Schlagwörter

ASJC Scopus Sachgebiete

Molecular Biology, Spectroscopy, Catalysis, Inorganic Chemistry, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/fccceb64-6bf5-489b-ba43-90324ba1d3a6