Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum

Autor(en)

Steven J. Hallam, Konstantinos T. Konstantinidis, Nik Putnam, Christa Schleper, Yoh-ichi Watanabe, Junichi Sugahara, Christina Preston, Jose de la Torre, Paul M. Richardson, Edward F. DeLong

Abstrakt

Crenarchaeota are ubiquitous and abundant microbial constituents of soils, sediments, lakes, and ocean waters. To further describe the cosmopolitan nonthermophilic Crenarchaeota, we analyzed the genome sequence of one representative, the uncultivated sponge symbiont Cenarchaeum symbiosum. C. symbiosum genotypes coinhabiting the same host partitioned into two dominant populations, corresponding to previously described a- and b-type ribosomal RNA variants. Although they were syntenic, overlapping a- and b-type ribotype genomes harbored significant variability. A single tiling path comprising the dominant a-type genotype was assembled and used to explore the genomic properties of C. symbiosum and its planktonic relatives. Of 2,066 ORFs, 55.6% matched genes with predicted function from previously sequenced genomes. The remaining genes partitioned between functional RNAs (2.4%) and hypotheticals (42%) with limited homology to known functional genes. The latter category included some genes likely involved in the archaeal¿sponge symbiotic association. Conversely, 525 C. symbiosum ORFs were most highly similar to sequences from marine environmental genomic surveys, and they apparently represent orthologous genes from free-living planktonic Crenarchaeota. In total, the C. symbiosum genome was remarkably distinct from those of other known Archaea and shared many core metabolic features in common with its free-living planktonic relatives.

Organisation(en)

Externe Organisation(en)

Massachusetts Institute of Technology, Monterey Bay Aquarium Research Institute, University of Tokyo, Keio University, University of Washington, United States Department of Energy

Journal

Proceedings of the National Academy of Sciences of the United States of America (PNAS)

Band

103

Seiten

18296-18301

Anzahl der Seiten

6

ISSN

0027-8424

Publikationsdatum

2006

Peer-reviewed

Ja

ÖFOS 2012

106013 Genetik

Sustainable Development Goals

SDG 14 – Leben unter Wasser

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/e7456b3c-46bb-4850-9223-2c10963b24d1