The Longitudinal Dividing Bacterium Candidatus Thiosymbion Oneisti Has a Natural Temperature-Sensitive FtsZ Protein with Low GTPase Activity

Autor(en)
Jinglan Wang, Silvia Bulgheresi, Tanneke den Blaauwen
Abstrakt

FtsZ, the bacterial tubulin-homolog, plays a central role in cell division and polymerizes into a ring-like structure at midcell to coordinate other cell division proteins. The rod-shaped gamma-proteobacterium Candidatus Thiosymbion oneisti has a medial discontinuous ellipsoidal “Z-ring.” Ca. T. oneisti FtsZ shows temperature-sensitive characteristics when it is expressed in Escherichia coli, where it localizes at midcell. The overexpression of Ca. T. oneisti FtsZ interferes with cell division and results in filamentous cells. In addition, it forms ring- and barrel-like structures independently of E. coli FtsZ, which suggests that the difference in shape and size of the Ca. T. oneisti FtsZ ring is likely the result of its interaction with Z-ring organizing proteins. Similar to some temperature-sensitive alleles of E. coli FtsZ, Ca. T. oneisti FtsZ has a weak GTPase and does not polymerize in vitro. The temperature sensitivity of Ca. Thiosymbion oneisti FtsZ is likely an adaptation to the preferred temperature of less than 30 °C of its host, the nematode Laxus oneistus.

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie
Externe Organisation(en)
Vrije Universiteit Amsterdam
Journal
International Journal of Molecular Sciences
Band
23
Anzahl der Seiten
15
ISSN
1422-0067
DOI
https://doi.org/10.3390/ijms23063016
Publikationsdatum
03-2022
Peer-reviewed
Ja
ÖFOS 2012
106022 Mikrobiologie
Schlagwörter
ASJC Scopus Sachgebiete
Molecular Biology, Spectroscopy, Catalysis, Inorganic Chemistry, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/the-longitudinal-dividing-bacterium-candidatus-thiosymbion-oneisti-has-a-natural-temperaturesensitive-ftsz-protein-with-low-gtpase-activity(d834e1a2-27d6-452f-973a-450c9de05a9b).html