Minimal and hybrid hydrogenases are active from archaea
- Autor(en)
- Chris Greening, Princess R Cabotaje, Luis E Valentin Alvarado, Pok Man Leung, Henrik Land, Thiago Rodrigues de Oliveira, Rafael I Ponce-Toledo, Moritz Senger, Max A Klamke, Michael Milton, Rachael Lappan, Susan Mullen, Jacob West-Roberts, Jie Mao, Jiangning Song, Marie Schoelmerich, Courtney W Stairs, Christa Schleper, Rhys Grinter, Anja Spang, Jillian F Banfield, Gustav Berggren
- Abstrakt
Microbial hydrogen (H2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H2. Moreover, we identify and characterize remarkable hybrid complexes formed through the fusion of [FeFe] and [NiFe] hydrogenases in ten other archaeal orders. Phylogenetic analysis and structural modeling suggest a deep evolutionary history of hybrid hydrogenases. These findings reveal new metabolic adaptations of archaea, streamlined H2 catalysts for biotechnological development, and a surprisingly intertwined evolutionary history between the two major H2-metabolizing enzymes.
- Organisation(en)
- Department für Funktionelle und Evolutionäre Ökologie
- Externe Organisation(en)
- Monash University, Uppsala University, University of California, Berkeley, Lund University, Royal Netherlands Institute for Sea Research, University of Amsterdam (UvA)
- Journal
- Cell
- Band
- 187
- Seiten
- 3357-3372.e19
- ISSN
- 0092-8674
- DOI
- https://doi.org/10.1016/j.cell.2024.05.032
- Publikationsdatum
- 06-2024
- Peer-reviewed
- Ja
- ÖFOS 2012
- 106022 Mikrobiologie
- Schlagwörter
- ASJC Scopus Sachgebiete
- Allgemeine Biochemie, Genetik und Molekularbiologie
- Link zum Portal
- https://ucrisportal.univie.ac.at/de/publications/a6771fba-3cbd-44c4-942a-8a74980f861f