Minimal and hybrid hydrogenases are active from archaea

Autor(en)

Chris Greening, Princess R Cabotaje, Luis E Valentin Alvarado, Pok Man Leung, Henrik Land, Thiago Rodrigues de Oliveira, Rafael I Ponce-Toledo, Moritz Senger, Max A Klamke, Michael Milton, Rachael Lappan, Susan Mullen, Jacob West-Roberts, Jie Mao, Jiangning Song, Marie Schoelmerich, Courtney W Stairs, Christa Schleper, Rhys Grinter, Anja Spang, Jillian F Banfield, Gustav Berggren

Abstrakt

Microbial hydrogen (H2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H2. Moreover, we identify and characterize remarkable hybrid complexes formed through the fusion of [FeFe] and [NiFe] hydrogenases in ten other archaeal orders. Phylogenetic analysis and structural modeling suggest a deep evolutionary history of hybrid hydrogenases. These findings reveal new metabolic adaptations of archaea, streamlined H2 catalysts for biotechnological development, and a surprisingly intertwined evolutionary history between the two major H2-metabolizing enzymes.

Organisation(en)

Department für Funktionelle und Evolutionäre Ökologie

Externe Organisation(en)

Uppsala University, University of California, Berkeley, Monash University, Lund University, Royal Netherlands Institute for Sea Research, University of Amsterdam (UvA)

Journal

Cell

Band

187

Seiten

3357-3372.e19

ISSN

0092-8674

DOI

https://doi.org/10.1016/j.cell.2024.05.032

Publikationsdatum

06-2024

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie

Schlagwörter

ASJC Scopus Sachgebiete

Biochemistry, Genetics and Molecular Biology(all)

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/minimal-and-hybrid-hydrogenases-are-active-from-archaea(a6771fba-3cbd-44c4-942a-8a74980f861f).html