Minimal and hybrid hydrogenases are active from archaea

Autor(en)
Chris Greening, Princess R Cabotaje, Luis E Valentin Alvarado, Pok Man Leung, Henrik Land, Thiago Rodrigues de Oliveira, Rafael I Ponce-Toledo, Moritz Senger, Max A Klamke, Michael Milton, Rachael Lappan, Susan Mullen, Jacob West-Roberts, Jie Mao, Jiangning Song, Marie Schoelmerich, Courtney W Stairs, Christa Schleper, Rhys Grinter, Anja Spang, Jillian F Banfield, Gustav Berggren
Abstrakt

Microbial hydrogen (H2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H2. Moreover, we identify and characterize remarkable hybrid complexes formed through the fusion of [FeFe] and [NiFe] hydrogenases in ten other archaeal orders. Phylogenetic analysis and structural modeling suggest a deep evolutionary history of hybrid hydrogenases. These findings reveal new metabolic adaptations of archaea, streamlined H2 catalysts for biotechnological development, and a surprisingly intertwined evolutionary history between the two major H2-metabolizing enzymes.

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie
Externe Organisation(en)
Monash University, Uppsala University, University of California, Berkeley, Lund University, Royal Netherlands Institute for Sea Research, University of Amsterdam (UvA)
Journal
Cell
Band
187
Seiten
3357-3372.e19
ISSN
0092-8674
DOI
https://doi.org/10.1016/j.cell.2024.05.032
Publikationsdatum
06-2024
Peer-reviewed
Ja
ÖFOS 2012
106022 Mikrobiologie
Schlagwörter
ASJC Scopus Sachgebiete
Allgemeine Biochemie, Genetik und Molekularbiologie
Link zum Portal
https://ucrisportal.univie.ac.at/de/publications/a6771fba-3cbd-44c4-942a-8a74980f861f