Copper limiting threshold in the terrestrial ammonia oxidizing archaeon Nitrososphaera viennensis

Autor(en)

Carolina Reyes, Logan Hodgskiss, Oliver Baars, Melina Kerou, Barbara Bayer, Christa Schleper, Stephan Krämer

Abstrakt

Ammonia oxidizing archaea (AOA) inhabiting soils have a central role in the global nitrogen cycle. Copper (Cu) is central to many enzymes in AOA including ammonia monooxygenase (AMO), the enzyme involved in the first step of ammonia oxidation. This study explored the physiological response of the AOA soil isolate, Nitrososphaera viennensis (EN76T) to Cu-limiting conditions in order to approach its limiting threshold under laboratory conditions. The chelator TETA (1,4,8,11-tetraazacyclotetradecane N, N’, N’’, N’’’-tetraaceticacid hydrochloride hydrate) with selective affinity for Cu2+ was used to lower bioavailable Cu2+ in culture experiments as predicted by thermodynamic speciation calculations. Results show that N. viennensis is Cu-limited at concentrations ≤10-15 mol L-1 free Cu2+ compared to standard conditions (10-12 mol L-1). This Cu2+ limiting threshold is similar to pure cultures of denitrifying bacteria and other AOA and AOB inhabiting soils, freshwaters and sewage (<10-16 mol L-1), and lower than pure cultures of the marine AOA Nitrosopumilus maritimus (<10-12.7 mol L-1), which also possesses a high amount of Cu-dependent enzymes.

Organisation(en)

Department für Umweltgeowissenschaften

Externe Organisation(en)

North Carolina State University

Journal

Research in Microbiology

Band

171

Seiten

134-142

Anzahl der Seiten

9

ISSN

0923-2508

DOI

https://doi.org/10.1016/j.resmic.2020.01.003

Publikationsdatum

09-2019

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie, 105906 Umweltgeowissenschaften

Schlagwörter

ASJC Scopus Sachgebiete

Molecular Biology, Microbiology

Sustainable Development Goals

SDG 14 – Leben unter Wasser

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/a547e447-1bab-41fa-9594-398cf66612e5