Analysis of the first genome fragment from the marine sponge-associated, novel candidate phylum Poribacteria by environmental genomics

Autor(en)

Lars Fieseler, Achim Quaiser, Christa Schleper, Ute Hentschel

Abstrakt

The novel candidate phylum Poribacteria is specifically associated with several marine demosponge genera. Because no representatives of Poribacteria have been cultivated, an environmental genomic approach was used to gain insights into genomic properties and possibly physiological/functional features of this elusive candidate division. In a large-insert library harbouring an estimated 1.1 Gb of microbial community DNA from Aplysina aerophoba, a Poribacteria-positive 16S rRNA gene locus was identified. Sequencing and sequence annotation of the 39 kb size insert revealed 27 open reading frames (ORFs) and two genes for stable RNAs. The fragment exhibited an overall G+C content of 50.5% and a coding density of 86.1%. The 16S rRNA gene was unlinked from a conventional rrn operon. Its flanking regions did not show any synteny to other 16S rRNA encoding loci from microorganisms with unlinked rrn operons. Two of the predicted hypothetical proteins were highly similar to homologues from Rhodopirellula baltica. Furthermore, a novel kind of molybdenum containing oxidoreductase was predicted as well as a series of eight ORFs encoding for unusual transporters, channel or pore forming proteins. This environmental genomics approach provides, for the first time, genomic and, by inference, functional information on the so far uncultivated, sponge-associated candidate division Poribacteria

Organisation(en)

Externe Organisation(en)

Julius-Maximilians-Universität Würzburg, Technische Universität Darmstadt

Journal

Environmental Microbiology

Band

8

Seiten

612-24

Anzahl der Seiten

13

ISSN

1462-2912

DOI

https://doi.org/10.1111/j.1462-2920.2005.00937.x

Publikationsdatum

04-2006

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie

Schlagwörter

Sustainable Development Goals

SDG 14 – Leben unter Wasser

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/analysis-of-the-first-genome-fragment-from-the-marine-spongeassociated-novel-candidate-phylum-poribacteria-by-environmental-genomics(9b5ff3ac-195d-41c2-b45e-26fc3f8ca5cb).html