Scale-up of biomass production by Methanococcus maripaludis

Hayk Palabikyan, Aquilla Ruddyard, Lara Pomper, David Novak, Barbara Reischl, Simon K.-M. R. Rittmann

The development of a sustainable energy economy is one of the great challenges in the current times of climate crisis and growing energy demands. Industrial production of the fifth-generation biofuel methane by microorganisms has the potential to become a crucial biotechnological milestone of the post fossil fuel era. Therefore, reproducible cultivation and scale-up of methanogenic archaea (methanogens) is essential for enabling biomass generation for fundamental studies and for defining peak performance conditions for bioprocess development. This study provides a comprehensive revision of established and optimization of novel methods for the cultivation of the model organism Methanococcus maripaludis S0001. In closed batch mode, 0.05 L serum bottles cultures were gradually replaced by 0.4 L Schott bottle cultures for regular biomass generation, and the time for reaching peak optical density (OD<jats:sub/>578) values was reduced in half. In 1.5 L reactor cultures, various agitation, harvesting and transfer methods were compared resulting in a specific growth rate of 0.16 h<jats:sup/>−1 and the highest recorded OD<jats:sub/>578 of 3.4. Finally, a 300-fold scale-up from serum bottles was achieved by growing M. maripaludis for the first time in a 22 L stainless steel bioreactor with 15 L working volume. Altogether, the experimental approaches described in this study contribute to establishing methanogens as essential organisms in large-scale biotechnology applications, a crucial stage of an urgently needed industrial evolution toward sustainable biosynthesis of energy and high value products.

Department für Funktionelle und Evolutionäre Ökologie, Department für Mikrobiologie, Immunbiologie und Genetik
Externe Organisation(en)
Masaryk University
Frontiers in Microbiology
ÖFOS 2012
209003 Biotreibstoffe, 209002 Bioprozesstechnik, 106022 Mikrobiologie, 209006 Industrielle Biotechnologie
ASJC Scopus Sachgebiete
Microbiology (medical), Microbiology
Sustainable Development Goals
SDG 13 – Maßnahmen zum Klimaschutz
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