Scale-up of biomass production by Methanococcus maripaludis
- Autor(en)
- Hayk Palabikyan, Aquilla Ruddyard, Lara Pomper, David Novak, Barbara Reischl, Simon K.-M. R. Rittmann
- Abstrakt
The development of a sustainable energy economy is one of the great challenges in the current times of climate crisis and growing energy demands. Industrial production of the fifth-generation biofuel methane by microorganisms has the potential to become a crucial biotechnological milestone of the post fossil fuel era. Therefore, reproducible cultivation and scale-up of methanogenic archaea (methanogens) is essential for enabling biomass generation for fundamental studies and for defining peak performance conditions for bioprocess development. This study provides a comprehensive revision of established and optimization of novel methods for the cultivation of the model organism Methanococcus maripaludis S0001. In closed batch mode, 0.05 L serum bottles cultures were gradually replaced by 0.4 L Schott bottle cultures for regular biomass generation, and the time for reaching peak optical density (OD<jats:sub/>578) values was reduced in half. In 1.5 L reactor cultures, various agitation, harvesting and transfer methods were compared resulting in a specific growth rate of 0.16 h<jats:sup/>−1 and the highest recorded OD<jats:sub/>578 of 3.4. Finally, a 300-fold scale-up from serum bottles was achieved by growing M. maripaludis for the first time in a 22 L stainless steel bioreactor with 15 L working volume. Altogether, the experimental approaches described in this study contribute to establishing methanogens as essential organisms in large-scale biotechnology applications, a crucial stage of an urgently needed industrial evolution toward sustainable biosynthesis of energy and high value products.
- Organisation(en)
- Department für Funktionelle und Evolutionäre Ökologie, Department für Mikrobiologie, Immunbiologie und Genetik
- Externe Organisation(en)
- Masaryk University
- Journal
- Frontiers in Microbiology
- Band
- 13
- ISSN
- 1664-302X
- DOI
- https://doi.org/10.3389/fmicb.2022.1031131
- Publikationsdatum
- 11-2022
- Peer-reviewed
- Ja
- ÖFOS 2012
- 209003 Biotreibstoffe, 209002 Bioprozesstechnik, 106022 Mikrobiologie, 209006 Industrielle Biotechnologie
- Schlagwörter
- ASJC Scopus Sachgebiete
- Microbiology (medical), Microbiology
- Sustainable Development Goals
- SDG 13 – Maßnahmen zum Klimaschutz
- Link zum Portal
- https://ucrisportal.univie.ac.at/de/publications/8d194a44-9cdc-498b-a10d-a33d54552529