Elucidating the transcription cycle of the UV-inducible hyperthermophilic archaeal virus SSV1 by DNA microarrays

Autor(en)

Sabrina Fröls, Paul M.K. Gordon, Mayi Arcellana Panlilio, Christa Schleper, Christoph W. Sensen

Abstrakt

The spindle-shaped Sulfolobus virus SSV1 was the first of a series of unusual and uniquely shaped viruses isolated from hyperthermophilic Archaea. Using whole-genome microarrays we show here that the circular 15.5 kb DNA genome of SSV1 exhibits a chronological regulation of its transcription upon UV irradiation, reminiscent to the life cycles of bacteriophages and eukaryotic viruses. The transcriptional cycle starts with a small UV-specific transcript and continues with early transcripts on both its flanks. The late transcripts appear after the onset of viral replication and are extended to their full lengths towards the end of the approximately 8.5 h cycle. While we detected only small differences in genome-wide analysis of the host Sulfolobus solfataricus comparing infected versus uninfected strains, we found a marked difference with respect to the strength and speed of the general UV response of the host. Models for the regulation of the virus cycle, and putative functions of genes in SSV1 are presented

Organisation(en)

Externe Organisation(en)

University of Bergen (UiB), University of Calgary

Journal

Virology

Band

365

Seiten

48-59

Anzahl der Seiten

12

ISSN

0042-6822

DOI

https://doi.org/10.1016/j.virol.2007.03.033

Publikationsdatum

2007

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie

Schlagwörter

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/elucidating-the-transcription-cycle-of-the-uvinducible-hyperthermophilic-archaeal-virus-ssv1-by-dna-microarrays(7a69a01f-c140-4a2d-94ab-a97a1f189c0a).html