Characterization of DsrD and its interaction with the DsrAB dissimilatory sulfite reductase

Autor(en)

Ana C C Barbosa, Sofia S Venceslau, Delfim Ferreira, Sinje Neukirchen, Maria Filipa Baltazar de Lima de Sousa, Manuel N Melo, Inês A C Pereira

Abstrakt

Microbial dissimilatory sulfate reduction is a key process in the global sulfur and carbon cycles in anoxic ecosystems. In this anaerobic respiration, sulfate is phosphorylated and reduced to sulfite, which is further reduced to a DsrC-trisulfide by the dissimilatory sulfite reductase DsrAB. DsrD is a small protein that acts as an allosteric activator of DsrAB, increasing the efficiency of sulfite reduction. Here, we report a detailed study of DsrD and its interaction with DsrAB. Sequence similarity analyses show that there are three groups of DsrD in organisms with a reductive-type DsrAB. The protein regions involved in the DsrD-DsrAB interaction and activity-promoting effect were investigated through in vitro and in silico studies, including mutations of conserved DsrD residues. The results reveal that the conserved β-loop of DsrD is involved in the interaction, contributing to a better understanding of its mechanism of action.

Organisation(en)

Department für Funktionelle und Evolutionäre Ökologie

Externe Organisation(en)

Universidade Nova de Lisboa

Journal

Protein Science

Band

33

ISSN

0961-8368

DOI

https://doi.org/10.1002/pro.5222

Publikationsdatum

11-2024

Peer-reviewed

Ja

ÖFOS 2012

106014 Genomik

Schlagwörter

ASJC Scopus Sachgebiete

Molecular Biology, Biochemistry

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/775af210-8f7a-49ab-9f7e-0bda1cb789bf