Scale-Up of Dark Fermentative Biohydrogen Production by Artificial Microbial Co-Cultures

Autor(en)

Ipek Ergal, Elisa Zech, Nikola Hanišáková, Ivan Kushkevych, Werner Fuchs, Tomáš Vítěz, Monika Vítězová, Günther Bochmann, Simon Karl-Maria Rasso Rittmann

Abstrakt

As a renewable energy carrier, dark fermentative biohydrogen (H2) represents a promising future alternative to fossil fuels. Recently, the limited H2 yield of 4 moles of H2 per mole glucose, the so-called “Thauer limit”, was surpassed by a defined artificial consortium. In this article, we demonstrate the upscaling of this drawing board design, from serum bottles to laboratory scale bioreactors. Our results illustrate that this designed microbial co-culture can be successfully implemented in batch mode, with maximum H2 yields of 6.18 and 4.45 mol mol−1 substrate. Furthermore, we report volumetric H2 productivities of 105.6 and 80.8 mmol H2 L−1 h−1. These rates are higher than for any other dark fermentative H2 production system using a synthetic microbial co-culture applied in batch mode on a defined medium. Our study is an important step forward for the application of artificial microbial consortia in future biotechnology and energy production systems.

Organisation(en)

Department für Funktionelle und Evolutionäre Ökologie

Externe Organisation(en)

Masaryk University, Universität für Bodenkultur Wien

Journal

Journal of Applied Microbiology

Band

2

Seiten

215-226

Anzahl der Seiten

12

ISSN

1364-5072

DOI

https://doi.org/10.3390/applmicrobiol2010015

Publikationsdatum

03-2022

Peer-reviewed

Ja

ÖFOS 2012

209005 Fermentation

Schlagwörter

ASJC Scopus Sachgebiete

Applied Microbiology and Biotechnology, Chemical Engineering (miscellaneous), Biochemistry, Genetics and Molecular Biology (miscellaneous)

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/5e08795b-18b2-4db9-ad39-3946c6c1b71e