Response of the hyperthermophilic archaeon Sulfolobus solfataricus to UV damage

Autor(en)

Sabrina Fröls, Paul M.K. Gordon, Mayi Arcellana Panlilio, Iain G. Duggin, Stephen D. Bell, Christoph W. Sensen, Christa Schleper

Abstrakt

In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements. While no significant up-regulation of genes potentially involved in direct DNA damage reversal was observed, a specific transcriptional UV response involving 55 genes could be dissected. Although flow cytometry showed only modest perturbation of the cell cycle, strong modulation of the transcript levels of the Cdc6 replication initiator genes was observed. Up-regulation of an operon encoding Mre11 and Rad50 homologs pointed to induction of recombinational repair. Consistent with this, DNA double-strand breaks were observed between 2 and 8 h after UV treatment, possibly resulting from replication fork collapse at damaged DNA sites. The strong transcriptional induction of genes which potentially encode functions for pilus formation suggested that conjugational activity might lead to enhanced exchange of genetic material. In support of this, a statistical microscopic analysis demonstrated that large cell aggregates formed upon UV exposure. Together, this

Organisation(en)

Externe Organisation(en)

University of Bergen (UiB), University of Calgary, MRC Cancer Cell Unit, University of Cambridge

Journal

Journal of Bacteriology

Band

189

Seiten

8708-8718

Anzahl der Seiten

11

ISSN

0021-9193

Publikationsdatum

2007

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/37451541-4e4a-4830-a57d-7a8e50b2710a