Host Langerin (CD207) is a receptor for Yersinia pestis phagocytosis and promotes dissemination

Autor(en)

K Yang, CG Park, C Cheong, Silvia Bulgheresi, Shusheng Zhang, Pei Zhang, Y He, L Jiang, H Huang, H Ding, Y Wu, S Wang, L Zhang, A Li, L Xia, SS Bartra, GV Plano, M Skurnik, J Klena, T Chen

Abstrakt

Yersinia pestis is a Gram-negative bacterium that causes plague. After Y. pestis overcomes the skin barrier, it encounters antigen-presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium-dependent (C-type) lectin. Furthermore, Y. pestis possesses exposed core oligosaccharides. In this study, we show that Y. pestis invades LCs and Langerin-expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, Y. pestis propensity to invade Langerhans and Langerin-expressing cells decreases. Moreover, the interaction of Y. pestis with Langerin-expressing transfectants is inhibited by purified Langerin, a DC-SIGN (DC-specific intercellular adhesion molecule 3 grabbing nonintegrin)-like molecule, an anti-CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of Y. pestis to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by Y. pestis. Therefore, Langerin-mediated binding of Y. pestis to APCs may promote its dissemination and infection.

Organisation(en)

Externe Organisation(en)

Huazhong University of Science and Technology, Institut de Recherches Cliniques de Montréal (IRCM), Chinese Center for Disease Control & Prevention, University of Miami, University of Helsinki, Peking University, University of Illinois at Chicago, Yonsei University

Journal

Immunology and cell biology

Band

93

Seiten

815-824

Anzahl der Seiten

10

ISSN

0818-9641

DOI

https://doi.org/10.1038/icb.2015.46

Publikationsdatum

10-2015

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie

Schlagwörter

ASJC Scopus Sachgebiete

Immunology and Allergy, Cell Biology, Immunology

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/343881f2-6d2c-4356-8bbc-a3e7d7c11631