A Simple and Straightforward Method for Activity Measurement of Carbonic Anhydrases

Autor(en)

Werner Fuchs, Franziska Steger, Johanna Reich, Doris Ribitsch, Simon K-M R. Rittmann, Guenther Bochmann

Abstrakt

Carbonic anhydrase (CA) is an enzyme of high interest due to its high implications relative to the medical and environmental sectors. In the current paper, an enzyme assay for the determination of CA activity is proposed and it is characterized by its simplicity and high practicability. It permits the straightforward comparison of CAs performance in physiological conditions. The methodology and the theoretical background of the evaluation method are explained in detail. Moreover, the presumed advantages over alternative assays are discussed. The assay has proven to be particularly useful for the screening of CA activity with respect to their application in CO2, capturing processes for further utilization or storage

Organisation(en)

Department für Funktionelle und Evolutionäre Ökologie

Externe Organisation(en)

Universität für Bodenkultur Wien, acib – Austrian Centre of Industrial Biotechnology

Journal

Catalysts

Band

11

Anzahl der Seiten

11

DOI

https://doi.org/10.3390/catal11070819

Publikationsdatum

07-2021

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie

Schlagwörter

ASJC Scopus Sachgebiete

Catalysis, Physical and Theoretical Chemistry

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/3201e2ca-a663-4eeb-9f4a-005fbcefb6b6