Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases

Autor(en)
Ivan Kushkevych, Kristýna Martínková, Lenka Mráková, Francesco Giudici, Simone Baldi, David Novak, Márió Gajdács, Monika Vítězová, Dani Dordevic, Amedeo Amedei, Simon K-M R Rittmann
Abstrakt

Considerable evidence has accumulated regarding the molecular relationship between gut microbiota (GM) composition and the onset (clinical presentation and prognosis of ulcerative colitis (UC)). In addition, it is well documented that short-chain fatty acid (SCFA)-producing bacteria may play a fundamental role in maintaining an anti-inflammatory intestinal homeostasis, but sulfate- and sulfite reducing bacteria may be responsible for the production of toxic metabolites, such as hydrogen sulfide and acetate. Hence, the present study aimed to assess the GM composition - focusing on sulfate-reducing bacteria (SRB) - in patients with severe, severe-active and moderate UC. Each one of the six enrolled patients provided two stool samples in the following way: one sample was cultivated in a modified SRB-medium before 16S rRNA sequencing and the other was not cultivated. Comparative phylogenetic analysis was conducted on each sample. Percentage of detected gut microbial genera showed considerable variation based on the patients' disease severity and cultivation in the SRB medium. In detail, samples without cultivation from patients with moderate UC showed a high abundance of the genera Bacteroides, Bifidobacterium and Ruminococcus, but after SRB cultivation, the dominant genera were Bacteroides, Klebsiella and Bilophila. On the other hand, before SRB cultivation, the main represented genera in patients with severe UC were Escherichia-Shigella, Proteus, Methanothermobacter and Methanobacterium. However, after incubation in the SRB medium Bacteroides, Proteus, Alistipes and Lachnoclostridium were predominant. Information regarding GM compositional changes in UC patients may aid the development of novel therapeutic strategies (e.g., probiotic preparations containing specific bacterial strains) to counteract the mechanisms of virulence of harmful bacteria and the subsequent inflammatory response that is closely related to the pathogenesis of inflammatory bowel diseases.

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie
Externe Organisation(en)
Masaryk University, Università degli Studi di Firenze, University of Szeged, Veterinärmedizinische und Pharmazeutische Universität Brünn
Journal
Microbial Cell
Band
11
Seiten
79-89
Anzahl der Seiten
11
ISSN
2311-2638
DOI
https://doi.org/10.15698/mic2024.03.817
Publikationsdatum
03-2024
Peer-reviewed
Ja
ÖFOS 2012
106022 Mikrobiologie
Schlagwörter
ASJC Scopus Sachgebiete
Applied Microbiology and Biotechnology, Genetics, Biochemistry, Genetics and Molecular Biology (miscellaneous), Molecular Biology, Virology, Parasitology, Cell Biology, Microbiology
Link zum Portal
https://ucrisportal.univie.ac.at/de/publications/comparison-of-microbial-communities-and-the-profile-of-sulfatereducing-bacteria-in-patients-with-ulcerative-colitis-and-their-association-with-bowel-diseases(0a6cc6e4-89ed-4f63-b17f-819e22cca482).html