Intestinal Epithelial Cell Tyrosine Kinase 2 Transduces IL-22 Signals To Protect from Acute Colitis

Autor(en)
Eva Hainzl, Silvia Stockinger, Isabella Rauch, Susanne Heider, David Berry, Caroline Lassnig, Clarissa Schwab, Felix Rosebrock, Gabriel Milinovich, Michaela Schlederer, Michael Wagner, Christa Schleper, Alexander Loy, Tim Urich, Lukas Kenner, Xiaonan Han, Thomas Decker, Birgit Strobl, Mathias Müller
Abstrakt

In the intestinal tract, IL-22 activates STAT3 to promote intestinal epithelial cell (IEC) homeostasis and tissue healing. The mechanism has remained obscure, but we demonstrate that IL-22 acts via tyrosine kinase 2 (Tyk2), a member of the Jak family. Using a mouse model for colitis, we show that Tyk2 deficiency is associated with an altered composition of the gut microbiota and exacerbates inflammatory bowel disease. Colitic Tyk2(-/-) mice have less p-STAT3 in colon tissue and their IECs proliferate less efficiently. Tyk2-deficient primary IECs show reduced p-STAT3 in response to IL-22 stimulation, and expression of IL-22-STAT3 target genes is reduced in IECs from healthy and colitic Tyk2(-/-) mice. Experiments with conditional Tyk2(-/-) mice reveal that IEC-specific depletion of Tyk2 aggravates colitis. Disease symptoms can be alleviated by administering high doses of rIL-22-Fc, indicating that Tyk2 deficiency can be rescued via the IL-22 receptor complex. The pivotal function of Tyk2 in IL-22-dependent colitis was confirmed in Citrobacter rodentium-induced disease. Thus, Tyk2 protects against acute colitis in part by amplifying inflammation-induced epithelial IL-22 signaling to STAT3.

Organisation(en)
Department für Mikrobiologie, Immunbiologie und Genetik, Department für Mikrobiologie und Ökosystemforschung, Forschungsverbund Chemistry Meets Microbiology, Großgeräteeinrichtung für Isotopenforschung, Department für Ökogenomik und Systembiologie
Journal
Journal of Immunology
Band
195
Seiten
5011-5024
Anzahl der Seiten
14
ISSN
0022-1767
DOI
https://doi.org/10.4049/jimmunol.1402565
Publikationsdatum
11-2015
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ÖFOS 2012
106022 Mikrobiologie
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/intestinal-epithelial-cell-tyrosine-kinase-2-transduces-il22-signals-to-protect-from-acute-colitis(dc2af80f-d3f6-4b7e-9085-3efc05df7319).html