Gene expression of lactobacilli in murine forestomach biofilms

Autor(en)

Clarissa Schwab, Alexander Tveit, Christa Schleper, Tim Urich

Abstrakt

Lactobacilli populate the gastro-intestinal tract of vertebrates, and are used in food fermentations and as probiotics. Lactobacilli are also major constituents of stable biofilms in the forestomach of rodents. In order to investigate the lifestyle of these biofilm lactobacilli in C57BL/6 mice, we applied metatranscriptomics to analyse gene expression (assessed by mRNA) and community composition (assessed by rRNA). Lactobacillales were the major biofilm inhabitants (62–82% of rRNA reads), followed by Clostridiales (8–31% of rRNA reads). To identify mRNA transcripts specific for the forestomach, we compared forestomach and hindgut metatranscriptomes. Gene expression of the biofilm microbiota was characterized by high abundance of transcripts related to glucose and maltose utilization, peptide degradation, and amino acid transport, indicating their major catabolic and anabolic pathways. The microbiota transcribed genes encoding pathways enhancing oxidative stress (glutathione synthesis) and acid tolerance. Various pathways, including metabolite formation (urea degradation, arginine pathway, γ-aminobutyrate) and cell wall modification (DltA, cyclopropane-fatty-acyl-phospholipid synthase), contributed to acid tolerance, as judged from the transcript profile. In addition, the biofilm microbiota expressed numerous genes encoding extracellular proteins involved in adhesion and/or biofilm formation (e.g. MucBP, glycosyl hydrolase families 68 and 70). This study shed light on the lifestyle and specific adaptations of lactobacilli in the murine forestomach that might also be relevant for lactobacilli biofilms in other vertebrates, including humans.

Organisation(en)

Externe Organisation(en)

University of Tromsø - The Arctic University of Norway

Journal

Microbial Biotechnology

Band

7

Seiten

347-359

Anzahl der Seiten

13

ISSN

1751-7915

DOI

https://doi.org/10.1111/1751-7915.12126

Publikationsdatum

04-2014

Peer-reviewed

Ja

ÖFOS 2012

106022 Mikrobiologie

ASJC Scopus Sachgebiete

Applied Microbiology and Biotechnology, Bioengineering, Biochemistry, Biotechnology

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/gene-expression-of-lactobacilli-in-murine-forestomach-biofilms(d0d0975b-cf0a-4e93-afbf-d0b78e5d69d4).html