FtsZ-mediated fission of a cuboid bacterial symbiont

Autor(en)
Philipp M. Weber, Gabriela F. Paredes, Tobias Viehboeck, Nika Pende, Jean Marie Volland, Olivier Gros, Michael VanNieuwenhze, Jörg Ott, Silvia Bulgheresi
Abstrakt

Less than a handful of cuboid and squared cells have been described in nature, which makes them a rarity. Here, we show how Candidatus Thiosymbion cuboideus, a cube-like gammaproteobacterium, reproduces on the surface of marine free-living nematodes. Immunostaining of symbiont cells with an anti-fimbriae antibody revealed that they are host-polarized, as these appendages exclusively localized at the host-proximal (animal-attached) pole. Moreover, by applying a fluorescently labeled metabolic probe to track new cell wall insertion in vivo, we observed that the host-attached pole started septation before the distal one. Similarly, Ca. T. cuboideus cells immunostained with an anti-FtsZ antibody revealed a proximal-to-distal localization pattern of this tubulin homolog. Although FtsZ has been shown to arrange into squares in synthetically remodeled cuboid cells, here we show that FtsZ may also mediate the division of naturally occurring ones. This implies that, even in natural settings, membrane roundness is not required for FtsZ function.

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie, Department für Mikrobiologie und Ökosystemforschung
Externe Organisation(en)
Institut Pasteur, United States Department of Energy, Indiana University Bloomington, LRC Systems, Université des Antilles et de la Guyane, Universität Wien
Journal
Iscience
Band
25
Anzahl der Seiten
14
ISSN
2589-0042
DOI
https://doi.org/10.1016/j.isci.2021.103552
Publikationsdatum
01-2022
Peer-reviewed
Ja
ÖFOS 2012
106022 Mikrobiologie
Schlagwörter
ASJC Scopus Sachgebiete
General
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/ftszmediated-fission-of-a-cuboid-bacterial-symbiont(c4055ce0-d099-45c6-82c2-a5a9ba6e5b27).html