Formate Utilization by the Crenarchaeon Desulfurococcus amylolyticus

Autor(en)

Ipek Ergal, Barbara Reischl, Benedikt Hasibar, Lokeshwaran Manoharan, Aaron Zipperle, Günther Bochmann, Werner Fuchs, Simon Karl-Maria Rasso Rittmann

Abstrakt

Formate is one of the key compounds of the microbial carbon and/or energy metabolism. It owes a significant contribution to various anaerobic syntrophic associations, and may become one of the energy storage compounds of modern energy biotechnology. Microbial growth on formate was demonstrated for different bacteria and archaea, but not yet for species of the archaeal phylum Crenarchaeota. Here, we show that Desulfurococcus amylolyticus DSM 16532, an anaerobic and hyperthermophilic Crenarchaeon, metabolises formate without the production of molecular hydrogen. Growth, substrate uptake, and production kinetics on formate, glucose, and glucose/formate mixtures exhibited similar specific growth rates and similar final cell densities. A whole cell conversion experiment on formate revealed that D. amylolyticus converts formate into carbon dioxide, acetate, citrate, and ethanol. Using bioinformatic analysis, we examined whether one of the currently known and postulated formate utilisation pathways could be operative in D. amylolyticus. This analysis indicated the possibility that D. amylolyticus uses formaldehyde producing enzymes for the assimilation of formate. Therefore, we propose that formate might be assimilated into biomass through formaldehyde dehydrogenase and the oxidative pentose phosphate pathway. These findings shed new light on the metabolic versatility of the archaeal phylum Crenarchaeota.

Organisation(en)

Department für Funktionelle und Evolutionäre Ökologie

Externe Organisation(en)

Universität für Bodenkultur Wien, Universität Wien

Journal

Microorganisms

Band

8

Anzahl der Seiten

20

DOI

https://doi.org/10.3390/microorganisms8030454

Publikationsdatum

03-2020

Peer-reviewed

Ja

ÖFOS 2012

209005 Fermentation

Schlagwörter

ASJC Scopus Sachgebiete

Microbiology (medical), Virology, Microbiology

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/formate-utilization-by-the-crenarchaeon-desulfurococcus-amylolyticus(bf0788f0-2f1e-4c0e-b1de-f9a7dbe186d8).html