Physiology and methane productivity of Methanobacterium thermaggregans

Autor(en)
Lisa-Maria Mauerhofer, Barbara Reischl, Tilman Schmider, Benjamin Schupp, Kinga Nagy, Patricia Pappenreiter, Sara Zwirtmayr, Bernhard Schuster, Sébastien Bernacchi, Arne H Seifert, Christian Paulik, Simon K-M R Rittmann
Abstrakt

Accumulation of carbon dioxide (CO2), associated with global temperature rise, and drastically decreasing fossil fuels necessitate the development of improved renewable and sustainable energy production processes. A possible route for CO2 recycling is to employ autotrophic and hydrogenotrophic methanogens for CO2-based biological methane (CH4) production (CO2-BMP). In this study, the physiology and productivity of Methanobacterium thermaggregans was investigated in fed-batch cultivation mode. It is shown that M. thermaggregans can be reproducibly adapted to high agitation speeds for an improved CH4 productivity. Moreover, inoculum size, sulfide feeding, pH, and temperature were optimized. Optimization of growth and CH4 productivity revealed that M. thermaggregans is a slightly alkaliphilic and thermophilic methanogen. Hitherto, it was only possible to grow seven autotrophic, hydrogenotrophic methanogenic strains in fed-batch cultivation mode. Here, we show that after a series of optimization and growth improvement attempts another methanogen, M. thermaggregas could be adapted to be grown in fed-batch cultivation mode to cell densities of up to 1.56 g L-1. Moreover, the CH4 evolution rate (MER) of M. thermaggregans was compared to Methanothermobacter marburgensis, the CO2-BMP model organism. Under optimized cultivation conditions, a maximum MER of 96.1 ± 10.9 mmol L-1 h-1 was obtained with M. thermaggregans-97% of the maximum MER that was obtained utilizing M. marburgensis in a reference experiment. Therefore, M. thermaggregans can be regarded as a CH4 cell factory highly suited to be applicable for CO2-BMP.

Organisation(en)
Department für Ökogenomik und Systembiologie
Journal
Applied Microbiology and Biotechnology
Band
102
Seiten
7643-7656
Anzahl der Seiten
14
ISSN
0175-7598
DOI
https://doi.org/10.1007/s00253-018-9183-2
Publikationsdatum
09-2018
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ÖFOS 2012
209003 Biotreibstoffe
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/physiology-and-methane-productivity-of-methanobacterium-thermaggregans(be8063ce-311b-43f7-9307-24310aaf1fec).html