Significance of dark CO2 fixation in arctic soils

Hana Santruckova, Petr Kotas, Jiri Barta, Tim Urich, Petr Capek, Juri Palmtag, Ricardo J. Eloy Alves, Christina Biasi, Katerina Diakova, Norman Gentsch, Antje Gittel, Georg Guggenberger, Gustaf Hugelius, Nikolaj Lashchinsky, Pertti J. Martikainen, Robert Mikutta, Christa Schleper, Joerg Schnecker, Clarissa Schwab, Olga Shibistova, Birgit Wild, Andreas Richter

The occurrence of dark fixation of CO2 by heterotrophic microorganisms in soil is generally accepted, but its importance for microbial metabolism and soil organic carbon (C) sequestration is unknown, especially under C limiting conditions. To fill this knowledge gap, we measured dark (CO2)-C-13 incorporation into soil organic matter and conducted a C-13-labelling experiment to follow the C-13 incorporation into phospholipid fatty acids as microbial biomass markers across soil profiles of four tundra ecosystems in the northern circumpolar region, where net primary productivity and thus soil C inputs are low. We further determined the abundance of various carboxylase genes and identified their microbial origin with metagenomics. The microbial capacity for heterotrophic CO2 fixation was determined by measuring the abundance of carboxylase genes and the incorporation of C-13 into soil C following the augmentation of bioavailable C sources. We demonstrate that dark CO2 fixation occurred ubiquitously in arctic tundra soils, with increasing importance in deeper soil horizons, presumably due to increasing C limitation with soil depth. Dark CO2 fixation accounted on average for 0.4, 1.0, 1.1, and 16% of net respiration in the organic, cryoturbated organic, mineral and permafrost horizons, respectively. Genes encoding anaplerotic enzymes of heterotrophic microorganisms comprised the majority of identified carboxylase genes. The genetic potential for dark CO2 fixation was spread over a broad taxonomic range. The results suggest important regulatory function of CO2 fixation in C limited conditions. The measurements were corroborated by modeling the long-term impact of dark CO2 fixation on soil organic matter. Our results suggest that increasing relative CO2 fixation rates in deeper soil horizons play an important role for soil internal C cycling and can, at least in part, explain the isotopic enrichment with soil depth.

Department für Ökogenomik und Systembiologie
Externe Organisation(en)
University of South Bohemia in České Budějovice , Ernst Moritz Arndt Universität Greifswald, Stockholm University, University of Eastern Finland, Gottfried Wilhelm Leibniz Universität Hannover, University of Bergen (UiB), Russian Academy of Sciences, Martin-Luther-Universität Halle-Wittenberg, University of New Hampshire, Austrian Polar Research Institute (APRI), Eidgenössische Technische Hochschule Zürich
Soil Biology and Biochemistry
Anzahl der Seiten
ÖFOS 2012
Mikrobiologie, Ökosystemforschung
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