Development of a simultaneous bioreactor system for characterization of gas production kinetics of methanogenic archaea at high pressure

Autor(en)
Patricia Pappenreiter, Sara Zwirtmayr, Lisa-Maria Mauerhofer, Simon Karl-Maria Rasso Rittmann, Christian Paulik
Abstrakt

Cultivation of methanogens under high pressure offers a great opportunity in biotechnological processes, one of which is the improvement of the gas‐liquid transfer of substrate gases into the medium broth. This article describes a newly developed simultaneous bioreactor system consisting of four identical cultivation vessels suitable for investigation of microbial activity at pressures up to 50 bar and temperatures up to 145°C. Initial pressure studies at 10 and 50 bar of the autotrophic and hydrogenotrophic methanogens Methanothermobacter marburgensis, Methanobacterium palustre, and Methanobacterium thermaggregans were performed to evaluate the reproducibility of the system as well as to test the productivity of these strains. The strains were compared with respect to gas conversion (%), methane evolution rate (MER) (mmol L‐1 h−1), turnover rate (h−1), and maximum conversion rate (kmin) (bar h−1). A pressure drop that can be explained by the reaction stoichiometry showed that all tested strains were active under pressurized conditions. Our study sheds light on the production kinetics of methanogenic strains under high‐pressure conditions. In addition, the simultaneous bioreactor system is a suitable first step screening system for analyzing the substrate uptake and/or production kinetics of gas conversion and/or gas production processes for barophilic or barotolerant microbes.

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie, Department für Ökogenomik und Systembiologie
Externe Organisation(en)
Johannes Kepler Universität Linz
Journal
Engineering in Life Sciences
Band
19
Seiten
537-544
ISSN
1618-0240
DOI
https://doi.org/10.1002/elsc.201900035
Publikationsdatum
2019
Peer-reviewed
Ja
ÖFOS 2012
Fermentation, Biotreibstoffe
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/development-of-a-simultaneous-bioreactor-system-for-characterization-of-gas-production-kinetics-of-methanogenic-archaea-at-high-pressure(5e744e12-4719-4fb3-bd88-803b21ed8223).html