Hyperthermophilic methanogenic archaea act as high-pressure CH4 cell factories

Autor(en)
Lisa-Maria Mauerhofer, Sara Zwirtmayr, Patricia Pappenreiter, Sébastien Bernacchi, Arne H Seifert, Barbara Reischl, Tilman Schmider, Ruth-Sophie Taubner, Christian Paulik, Simon K-M R Rittmann
Abstrakt

Bioprocesses converting carbon dioxide with molecular hydrogen to methane (CH4) are currently being developed to enable a transition to a renewable energy production system. In this study, we present a comprehensive physiological and biotechnological examination of 80 methanogenic archaea (methanogens) quantifying growth and CH4 production kinetics at hyperbaric pressures up to 50 bar with regard to media, macro-, and micro-nutrient supply, specific genomic features, and cell envelope architecture. Our analysis aimed to systematically prioritize high-pressure and high-performance methanogens. We found that the hyperthermophilic methanococci Methanotorris igneus and Methanocaldococcoccus jannaschii are high-pressure CH4 cell factories. Furthermore, our analysis revealed that high-performance methanogens are covered with an S-layer, and that they harbour the amino acid motif Tyrα444 Glyα445 Tyrα446 in the alpha subunit of the methyl-coenzyme M reductase. Thus, high-pressure biological CH4 production in pure culture could provide a purposeful route for the transition to a carbon-neutral bioenergy sector.

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie
Externe Organisation(en)
Johannes Kepler Universität Linz, Krajete GmbH, Austria
Journal
Communications Biology
Band
4
ISSN
2399-3642
DOI
https://doi.org/10.1038/s42003-021-01828-5
Publikationsdatum
03-2021
Peer-reviewed
Ja
ÖFOS 2012
209005 Fermentation
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/hyperthermophilic-methanogenic-archaea-act-as-highpressure-ch4-cell-factories(5bcb7a51-d8b8-4da7-9230-94b0f862d373).html