The Historical Development of Cultivation Techniques for Methanogens and Other Strict Anaerobes and Their Application in Modern Microbiology

Autor(en)
Nikola Hanišáková, Monika Vítězová, Simon K-M R Rittmann
Abstrakt

The cultivation and investigation of strictly anaerobic microorganisms belong to the fields of anaerobic microbial physiology, microbiology, and biotechnology. Anaerobic cultivation methods differ from classic microbiological techniques in several aspects. The requirement for special instruments, which are designed to prevent the contact of the specimen with air/molecular oxygen by different means of manipulation, makes this field more challenging for general research compared to working with aerobic microorganisms. Anaerobic microbiological methods are required for many purposes, such as for the isolation and characterization of new species and their physiological examination, as well as for anaerobic biotechnological applications or medical indications. This review presents the historical development of methods for the cultivation of strictly anaerobic microorganisms focusing on methanogenic archaea, anaerobic cultivation methods that are still widely used today, novel methods for anaerobic cultivation, and almost forgotten, but still relevant, techniques

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie
Externe Organisation(en)
Masaryk University
Journal
Microorganisms
Band
10
Anzahl der Seiten
28
ISSN
2076-2607
DOI
https://doi.org/10.3390/microorganisms10020412
Publikationsdatum
02-2022
Peer-reviewed
Ja
ÖFOS 2012
209005 Fermentation
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/the-historical-development-of-cultivation-techniques-for-methanogens-and-other-strict-anaerobes-and-their-application-in-modern-microbiology(3fae0f6a-da2f-4c1b-b073-5542f8b4776d).html