Host Langerin (CD207) is a receptor for Yersinia pestis phagocytosis and promotes dissemination

Autor(en)
K Yang, CG Park, C Cheong, Silvia Bulgheresi, Shusheng Zhang, Pei Zhang, Y He, L Jiang, H Huang, H Ding, Y Wu, S Wang, L Zhang, A Li, L Xia, SS Bartra, GV Plano, M Skurnik, J Klena, T Chen
Abstrakt

Yersinia pestis is a Gram-negative bacterium that causes plague. After Y. pestis overcomes the skin barrier, it encounters antigen-presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium-dependent (C-type) lectin. Furthermore, Y. pestis possesses exposed core oligosaccharides. In this study, we show that Y. pestis invades LCs and Langerin-expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, Y. pestis propensity to invade Langerhans and Langerin-expressing cells decreases. Moreover, the interaction of Y. pestis with Langerin-expressing transfectants is inhibited by purified Langerin, a DC-SIGN (DC-specific intercellular adhesion molecule 3 grabbing nonintegrin)-like molecule, an anti-CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of Y. pestis to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by Y. pestis. Therefore, Langerin-mediated binding of Y. pestis to APCs may promote its dissemination and infection.

Organisation(en)
Department für Ökogenomik und Systembiologie
Journal
Immunology and cell biology
Band
93
Seiten
815-824
ISSN
0818-9641
DOI
https://doi.org/10.1038/icb.2015.46
Publikationsdatum
2015
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ÖFOS 2012
106022 Mikrobiologie
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/host-langerin-cd207-is-a-receptor-for-yersinia-pestis-phagocytosis-and-promotes-dissemination(343881f2-6d2c-4356-8bbc-a3e7d7c11631).html