Indications for a moonlighting function of translation factor aIF5A in the crenarchaeum Sulfolobus solfataricus

Autor(en)
Flavia Bassani, Isabelle Anna Zink, Thomas Pribasnig, Michael T. Wolfinger, Alice Romagnoli, Armin Resch, Christa Schleper, Udo Bläsi, Anna La Teana
Abstrakt

Translation factor a/eIF5A is highly conserved in Eukarya and Archaea. The eukaryal eIF5A protein is required for transit of ribosomes across consecutive proline codons, whereas the function of the archaeal orthologue remains unknown. Here, we provide a first hint for an involvement of Sulfolobus solfataricus (Sso) aIF5A in translation. CRISPR-mediated knock down of the aif5A gene resulted in strong growth retardation, underlining a pivotal function. Moreover, in vitro studies revealed that Sso aIF5A is endowed with endoribonucleolytic activity. Thus, aIF5A appears to be a moonlighting protein that might be involved in protein synthesis as well as in RNA metabolism.

Organisation(en)
Department für Mikrobiologie, Immunbiologie und Genetik, Department für Ökogenomik und Systembiologie, Forschungsgruppe Bioinformatics and Computational Biology, Institut für Theoretische Chemie
Externe Organisation(en)
Max F. Perutz Laboratories GmbH (MFPL), Università Politecnica delle Marche
Journal
RNA Biology
Band
16
Seiten
675-685
Anzahl der Seiten
11
ISSN
1547-6286
DOI
https://doi.org/10.1080/15476286.2019.1582953
Publikationsdatum
05-2019
Peer-reviewed
Ja
ÖFOS 2012
Mikrobiologie, Biochemie, Molekularbiologie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/indications-for-a-moonlighting-function-of-translation-factor-aif5a-in-the-crenarchaeum-sulfolobus-solfataricus(310c1826-2f4a-4802-950e-af71f60cc7b3).html