Indications for a moonlighting function of translation factor aIF5A in the crenarchaeum Sulfolobus solfataricus

Autor(en)

Flavia Bassani, Isabelle Anna Zink, Thomas Pribasnig, Michael T. Wolfinger, Alice Romagnoli, Armin Resch, Christa Schleper, Udo Bläsi, Anna La Teana

Abstrakt

Translation factor a/eIF5A is highly conserved in Eukarya and Archaea. The eukaryal eIF5A protein is required for transit of ribosomes across consecutive proline codons, whereas the function of the archaeal orthologue remains unknown. Here, we provide a first hint for an involvement of Sulfolobus solfataricus (Sso) aIF5A in translation. CRISPR-mediated knock down of the aif5A gene resulted in strong growth retardation, underlining a pivotal function. Moreover, in vitro studies revealed that Sso aIF5A is endowed with endoribonucleolytic activity. Thus, aIF5A appears to be a moonlighting protein that might be involved in protein synthesis as well as in RNA metabolism.

Organisation(en)

Department für Mikrobiologie, Immunbiologie und Genetik, Department für Ökogenomik und Systembiologie, Forschungsgruppe Bioinformatics and Computational Biology, Institut für Theoretische Chemie

Externe Organisation(en)

Università Politecnica delle Marche, Max F. Perutz Laboratories GmbH (MFPL)

Journal

RNA Biology

Band

16

Seiten

675-685

Anzahl der Seiten

11

ISSN

1547-6286

DOI

https://doi.org/10.1080/15476286.2019.1582953

Publikationsdatum

05-2019

Peer-reviewed

Ja

ÖFOS 2012

Mikrobiologie, Biochemie, Molekularbiologie

Schlagwörter

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/indications-for-a-moonlighting-function-of-translation-factor-aif5a-in-the-crenarchaeum-sulfolobus-solfataricus(310c1826-2f4a-4802-950e-af71f60cc7b3).html