Longitudinal study of murine microbiota activity and interactions with the host during acute inflammation and recovery
- Autor(en)
- Clarissa Schwab, David Berry, Isabella Rauch, Ina Rennisch, Julia Ramesmayer, Eva Hainzl, Susanne Heider, Thomas Decker, Lukas Kenner, Mathias Müller, Birgit Strobl, Michael Wagner, Christa Schleper, Alexander Loy, Tim Urich
- Abstrakt
Although alterations in gut microbiota composition during acute colitis have been repeatedly observed, associated functional changes and the recovery from dysbiosis received little attention. In this study, we investigated structure and function of the gut microbiota during acute inflammation and recovery in a dextran sodium sulfate (DSS)-colitis mouse model using metatranscriptomics, bacterial 16S rRNA gene amplicon sequencing and monitoring of selected host markers. Parallel to an increase of host markers of inflammation during acute colitis, we observed relative abundance shifts and alterations in phylotype composition of the dominant bacterial orders Clostridiales and Bacteroidales, and an increase of the low abundant Enterobacteriales, Deferribacterales, Verrucomicrobiales and Erysipelotrichales. During recovery, the microbiota began to resume, but did not reach its original composition until the end of the experiment. Microbial gene expression was more resilient to disturbance, with pre-perturbation-type transcript profiles appearing quickly after acute colitis. The decrease of Clostridiales during inflammation correlated with a reduction of transcripts related to butyrate formation, suggesting a disturbance in host-microbe signalling and mucosal nutrient provision. The impact of acute inflammation on the Clostridiales was also characterized by a significant downregulation of their flagellin-encoding genes. In contrast, the abundance of members of the Bacteroidales increased along with an increase in transcripts related to mucin degradation. We propose that acute inflammation triggered a selective reaction of the immune system against flagella of commensals and temporarily altered murine microbiota composition and functions relevant for the host. Despite changes in specific interactions, the host-microbiota homeostasis revealed a remarkable ability for recovery.The ISME Journal advance online publication, 9 January 2014; doi:10.1038/ismej.2013.223.
- Organisation(en)
- Department für Mikrobiologie, Immunbiologie und Genetik
- Externe Organisation(en)
- Medizinische Universität Wien, Veterinärmedizinische Universität Wien
- Journal
- The ISME Journal: multidisciplinary journal of microbial ecology
- Band
- 8
- Seiten
- 1101-1114
- Anzahl der Seiten
- 14
- ISSN
- 1751-7362
- DOI
- https://doi.org/10.1038/ismej.2013.223
- Publikationsdatum
- 01-2014
- Peer-reviewed
- Ja
- ÖFOS 2012
- 106022 Mikrobiologie, 303020 Medizinische Mikrobiologie
- Schlagwörter
- ASJC Scopus Sachgebiete
- Ecology, Evolution, Behavior and Systematics, Microbiology
- Link zum Portal
- https://ucris.univie.ac.at/portal/de/publications/longitudinal-study-of-murine-microbiota-activity-and-interactions-with-the-host-during-acute-inflammation-and-recovery(f37e8538-f236-48f0-b483-e0bcad19f6ca).html