A Simple and Straightforward Method for Activity Measurement of Carbonic Anhydrases

Autor(en)
Werner Fuchs, Franziska Steger, Johanna Reich, Doris Ribitsch, Simon K-M R. Rittmann, Guenther Bochmann
Abstrakt

Carbonic anhydrase (CA) is an enzyme of high interest due to its high implications relative to the medical and environmental sectors. In the current paper, an enzyme assay for the determination of CA activity is proposed and it is characterized by its simplicity and high practicability. It permits the straightforward comparison of CAs performance in physiological conditions. The methodology and the theoretical background of the evaluation method are explained in detail. Moreover, the presumed advantages over alternative assays are discussed. The assay has proven to be particularly useful for the screening of CA activity with respect to their application in CO2, capturing processes for further utilization or storage

Organisation(en)
Department für Funktionelle und Evolutionäre Ökologie
Externe Organisation(en)
Universität für Bodenkultur Wien, acib – Austrian Centre of Industrial Biotechnology
Journal
Catalysts
Band
11
Anzahl der Seiten
11
DOI
https://doi.org/10.3390/catal11070819
Publikationsdatum
07-2021
Peer-reviewed
Ja
ÖFOS 2012
106022 Mikrobiologie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/a-simple-and-straightforward-method-for-activity-measurement-of-carbonic-anhydrases(3201e2ca-a663-4eeb-9f4a-005fbcefb6b6).html