A new C-type lectin similar to the human immunoreceptor DC-SIGN mediates symbiont acquisition by a marine nematode

Autoren:Bulgheresi, Silvia; Schabussova, Irma (University of Edinburgh); Chen, Tie (University of Illinois at Chicago); Mullin, N P (University of Edinburgh); Maizels, R M (University of Edinburgh); Ott, Jörg
Abstrakt:Although thiotrophic symbioses have been intensively studied for the last three decades, nothing is known about the molecular mechanisms of symbiont acquisition. We used the symbiosis between the marine nematode Laxus oneistus and sulfur-oxidizing bacteria to study this process. In this association a monolayer of symbionts covers the whole cuticle of the nematode, except its anterior-most region. Here, we identify a novel Ca2+-dependent mannose-specific lectin that was exclusively secreted onto the posterior, bacterium-associated region of L. oneistus cuticle. A recombinant form of this lectin induced symbiont aggregation in seawater and was able to compete with the native lectin for symbiont binding in vivo. Surprisingly, the carbohydrate recognition domain of this mannose-binding protein was similar both structurally and functionally to a human dendritic cell-specific immunoreceptor. Our results provide a molecular link between bacterial symbionts and host-secreted mucus in a marine symbiosis and suggest conservation in the mechanisms of host-microbe interactions throughout the animal kingdom. Copyright Π2006, American Society for Microbiology. All Rights Reserved.
Anzahl der Seiten:7
Journaltitel:Applied and Environmental Microbiology
Peer reviewed:true
Digital Object Identifier (DOI):http://dx.doi.org/10.1128/?AEM.72.4.2950-2956.2006
Bibliographische Notiz:***2370.28***Zeitschrift: Applied and Environmental Microbiology DOI: 10.1128/AEM.72.4.2950-2956.2006 Coden: AEMID Affiliations: Vienna Ecology Centre, Department of Marine Biology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria; Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh EH93JT, United Kingdom; Department of Biomedical Science, College of Medicine-Rockford, University of Illinois at Chicago, Rockford, IL, United States; Department of Chemistry, University of Edinburgh, Edinburgh EH93JT, United Kingdom Adressen: Bulgheresi, S.; Vienna Ecology Centre; Department of Marine Biology; University of Vienna; Althanstrasse 14 1090 Vienna, Austria; email: silvia.bulgheresi@univie.ac.at Import aus Scopus: 2-s2.0-33646079635 12.02.2008: Datenanforderung 2109 (Import Sachbearbeiter)