Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum

Autor(en)
Steven J. Hallam, Konstantinos T. Konstantinidis, Nik Putnam, Christa Schleper, Yoh-ichi Watanabe, Junichi Sugahara, Christina Preston, Jose de la Torre, Paul M. Richardson, Edward F. DeLong
Abstrakt

Crenarchaeota are ubiquitous and abundant microbial constituents of soils, sediments, lakes, and ocean waters. To further describe the cosmopolitan nonthermophilic Crenarchaeota, we analyzed the genome sequence of one representative, the uncultivated sponge symbiont Cenarchaeum symbiosum. C. symbiosum genotypes coinhabiting the same host partitioned into two dominant populations, corresponding to previously described a- and b-type ribosomal RNA variants. Although they were syntenic, overlapping a- and b-type ribotype genomes harbored significant variability. A single tiling path comprising the dominant a-type genotype was assembled and used to explore the genomic properties of C. symbiosum and its planktonic relatives. Of 2,066 ORFs, 55.6% matched genes with predicted function from previously sequenced genomes. The remaining genes partitioned between functional RNAs (2.4%) and hypotheticals (42%) with limited homology to known functional genes. The latter category included some genes likely involved in the archaeal¿sponge symbiotic association. Conversely, 525 C. symbiosum ORFs were most highly similar to sequences from marine environmental genomic surveys, and they apparently represent orthologous genes from free-living planktonic Crenarchaeota. In total, the C. symbiosum genome was remarkably distinct from those of other known Archaea and shared many core metabolic features in common with its free-living planktonic relatives.

Organisation(en)
Externe Organisation(en)
Massachusetts Institute of Technology, Monterey Bay Aquarium Research Institute, University of Tokyo, Keio University, University of Washington, United States Department of Energy
Journal
Proceedings of the National Academy of Sciences of the United States of America (PNAS)
Band
103
Seiten
18296-18301
Anzahl der Seiten
6
ISSN
0027-8424
Publikationsdatum
2006
Peer-reviewed
Ja
ÖFOS 2012
106013 Genetik
Sustainable Development Goals
SDG 14 – Leben unter Wasser
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/genomic-analysis-of-the-uncultivated-marine-crenarchaeote-cenarchaeum-symbiosum(e7456b3c-46bb-4850-9223-2c10963b24d1).html