Inhibitory effects of C2 to C10 1-alkynes on ammonia oxidation in two Nitrososphaera species

Autor(en)
A. E. Taylor, K. Taylor, B. Tennigkeit, Marton Palatinszky, Michaela Stieglmeier, D. D. Myrold, Christa Schleper, Michael Wagner, P. J. Bottomley
Abstrakt

A previous study showed that ammonia oxidation by the Thaumarchaeota Nitrosopumilus maritimus (group 1.1a) was resistant to concentrations of the C

8 1-alkyne, octyne, which completely inhibits activity by ammonia-oxidizing bacteria. In this study, the inhibitory effects of octyne and other C

2 to C

10 1-alkynes were evaluated on the nitrite production activity of two pure culture isolates from Thaumarchaeota group 1.1b, Nitrososphaera viennensis strain EN76 and Nitrososphaera gargensis. Both N. viennensis and N. gargensis were insensitive to concentrations of octyne that cause complete and irreversible inactivation of nitrite production by ammonia-oxidizing bacteria. However, octyne concentrations (≥20 μM) that did not inhibit N. maritimus partially inhibited nitrite production in N. viennensis and N. gargensis in a manner that did not show the characteristics of irreversible inactivation. In contrast to previous studies with an ammonia-oxidizing bacterium, Nitrosomonas europaea, octyne inhibition of N. viennensis was: (i) fully and immediately reversible, (ii) not competitive with NH

4

+, and (iii) without effect on the competitive interaction between NH

4

+ and acetylene. Both N. viennensis and N. gargensis demonstrated the same overall trend in regard to 1-alkyne inhibition as previously observed for N. maritimus, being highly sensitive to ≤C

5 alkynes and more resistant to longer-chain length alkynes. Reproducible differences were observed among N. maritimus, N. viennensis, and N. gargensis in regard to the extent of their resistance/sensitivity to C

6 and C

7 1-alkynes, which may indicate differences in the ammonia monooxygenase binding and catalytic site(s) among the Thaumarchaeota.

Organisation(en)
Externe Organisation(en)
Oregon State University
Journal
Applied and Environmental Microbiology
Band
81
Seiten
1942-1948
Anzahl der Seiten
7
ISSN
0099-2240
DOI
https://doi.org/10.1128/AEM.03688-14
Publikationsdatum
03-2015
Peer-reviewed
Ja
ÖFOS 2012
106022 Mikrobiologie
Schlagwörter
ASJC Scopus Sachgebiete
Applied Microbiology and Biotechnology, Food Science, Biotechnology, Ecology
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/inhibitory-effects-of-c2-to-c10-1alkynes-on-ammonia-oxidation-in-two-nitrososphaera-species(d4d7fd00-a757-406f-b707-52dfb4a2b7ff).html