A new C-type lectin similar to the human immunoreceptor DC-SIGN mediates symbiont acquisition by a marine nematode

Autor(en)
Silvia Bulgheresi , Irma Schabussova , Tie Chen , N P Mullin , R M Maizels , Jörg Ott
Abstrakt

Although thiotrophic symbioses have been intensively studied for the last three decades, nothing is known about the molecular mechanisms of symbiont acquisition. We used the symbiosis between the marine nematode Laxus oneistus and sulfur-oxidizing bacteria to study this process. In this association a monolayer of symbionts covers the whole cuticle of the nematode, except its anterior-most region. Here, we identify a novel Ca2+-dependent mannose-specific lectin that was exclusively secreted onto the posterior, bacterium-associated region of L. oneistus cuticle. A recombinant form of this lectin induced symbiont aggregation in seawater and was able to compete with the native lectin for symbiont binding in vivo. Surprisingly, the carbohydrate recognition domain of this mannose-binding protein was similar both structurally and functionally to a human dendritic cell-specific immunoreceptor. Our results provide a molecular link between bacterial symbionts and host-secreted mucus in a marine symbiosis and suggest conservation in the mechanisms of host-microbe interactions throughout the animal kingdom. Copyright Π2006, American Society for Microbiology. All Rights Reserved.

Organisation(en)
Journal
Applied and Environmental Microbiology
Band
72
Seiten
2950-2956
Anzahl der Seiten
7
ISSN
0099-2240
DOI
https://doi.org/10.1128/?AEM.72.4.2950-2956.2006
Publikationsdatum
2006
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ÖFOS 2012
106001 Allgemeine Biologie
ÖFOS 2002
1401 Allgemeine Biologie
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/a-new-ctype-lectin-similar-to-the-human-immunoreceptor-dcsign-mediates-symbiont-acquisition-by-a-marine-nematode(a42e11f0-314b-44ae-b3b4-f1b3cd400f3b).html